LYMPHOCYTE SUBSET PANEL WITH NK CELLS-(PANEL-1)

LYMPHOCYTE SUBSET PANEL WITH NK CELLS:Test Description

The Lymphocyte Subset Panel with NK Cells is a comprehensive blood test that quantifies various lymphocyte subsets, including T cells, B cells, and natural killer (NK) cells.

The Lymphocyte Subset Panel with NK Cells provides valuable insights into the immune system’s functionality and can assist in diagnosing immunodeficiencies, monitoring immune status in various diseases, and evaluating responses to therapies.

Principle

The test is based on flow cytometry, a technique that uses lasers to analyze the physical and chemical characteristics of cells. Fluorescent-labeled antibodies bind to specific cell surface markers, allowing the identification and quantification of lymphocyte subsets. This helps assess immune function and diagnose conditions like immunodeficiencies, autoimmune diseases, or lymphoproliferative disorders.

Test Components

This panel typically includes measurements for:

• CD3: Total T cells
• CD4: Helper T cells
• CD8: Cytotoxic T cells
• CD19: B cells
• CD56: Natural Killer (NK) cells

Procedure

1. Sample Collection: A blood sample is collected in a lavender-top EDTA tube to preserve the cells.
2. Preparation: The sample is processed to isolate lymphocytes.
3. Flow Cytometry: Fluorescent-labeled antibodies specific to surface markers (e.g., CD3, CD4, CD8, CD19, CD56) are added to the sample. These markers help identify and quantify the different lymphocyte subsets.
4. Analysis: The sample is run through a flow cytometer, which detects the fluorescence and provides data on the proportions and absolute counts of T cells, B cells, and NK cells.
5. Reporting: Results include percentages and absolute counts of each subset, as well as the CD4:CD8 ratio.

Conditions or Diseases that Require the Lymphocyte Subset Panel with NK Cells

 

• HIV/AIDS: Changes in CD4+ and CD8+ T cell numbers are crucial indicators of HIV disease progression and treatment response.
• Certain Cancers: Like lymphomas or leukemias.
• Autoimmune Diseases: Such as systemic lupus erythematosus (SLE) or rheumatoid arthritis, where the immune system might be overactive.
• Primary or Secondary Immunodeficiencies: Where there’s a suspected inherent weakness in the immune system or an acquired one due to medications or other conditions.

Indications for the Lymphocyte Subset Panel with NK Cells:

The Lymphocyte Subset Panel is indicated for:

• Evaluating patients with suspected immunodeficiency disorders.
• Monitoring individuals undergoing immunosuppressive therapy or those with autoimmune diseases.
• Assessing immune status in patients with viral infections, such as HIV.
• Investigating lymphoproliferative disorders.

Patient Preparation/Instructions

• Specimen Type: A blood sample is required, typically collected in a lavender top (EDTA) tube.
• Volume: A preferred volume of 3 to 5 mL is needed, with a minimum of 1 mL acceptable.
• Transport: Samples should be transported at room temperature and tested within 24 hours of collection to ensure accuracy.

Reference Values for Lymphocyte Subset Panel with NK Cells Test

Reference ranges can vary by laboratory but generally include:

• CD4 Count: 33-61% of total lymphocytes
• CD8 Count: 14-35% of total lymphocytes
• NK Cell Count (CD56): Varies based on individual health status

Stability Requirements

 

Turnaround Time for Lymphocyte Subset Panel with NK Cells

Results for the Lymphocyte Subset Panel with NK Cells typically take about 3 days to process, depending on the laboratory.

Most Common Questions About Lymphocyte Subset Panel  with NK Cells Test:

What is the primary objective of the Lymphocyte Subset Panel 1 Test?

The Lymphocyte Subset Panel with NK Cells primarily aims to measure and provide a count of different types of white blood cells, specifically T cells, B cells, and NK (natural killer) cells, which play a vital role in the body’s immune response.

When is the lymphocyte subset panel with NK cells test typically ordered by physicians?

The test is often ordered when a physician suspects an immune deficiency or an imbalance in the types of lymphocytes, especially in conditions like HIV/AIDS, certain types of leukemia, or following organ transplantation.

How To Order the Lymphocyte Subset Panel with NK Cells

To order the Lymphocyte Subset Panel with NK Cells, please click the ‘Order Test’ button and add it to your cart.

This test will provide essential information regarding your immune system’s health and functionality.

REFERENCE

http://www.labcorp.com/tests/505370/t-and-b-lymphocyte-and-natural-killer-cell-profilehttp://www.mayocliniclabs.com/test-catalog/Overview/9336http://testdirectory.questdiagnostics.com/test/test-detail/7197/lymphocyte-subset-panel-1?cc=MASTER